(625z) Binding and Dissociation of Biomarkers for Alzheimer's Disease On Biosensor Surfaces

A fractal analysis is presented for the binding and dissociation (if applicable) of Alzheimer’s disease biomarkers on biosensor surfaces. The Alzheimer’s disease biomarkers analyzed include: (a) the binding of different concentrations of APP₇₇₀ (amyloid precursor protein) in complete medium to the DE antibodies immobilized on a solid surface using total internal reflection elliposmetry (Mustafa et al., 2010), (b) binding of different concentrations of PrP (prion protein) variant, ARQ (in nM) in solution to asolectine liposome antibodies immobilized on a SPR biosensor chip (Steunou et al., 2010), (c) binding of different concentrations of O₃ (in nM) in solution to asolectine liposomes immobilized on the surface of a SPR biosensor chip (Steunou et al., 2010), (d) binding of 100 nM ARQ-Cu (II) to chip immobilized PC liposomes with or without lipid rafts (Steunou et al., 2010), (e) binding of different concentrations of Ab (in mM) in solution to sonicated fAb immobilized on a flow cell (Hasegawa et al., 2002), (f) binding of different concentrations of Ab (in mM) in solution to fAb immobilized on a flow cell (repetition runs) (Hasegawa et al., 2002), (g) binding of acetylcholine and AChE inhibitor to acetylcholinesterase and choline oxidase immobilized on a working electrode using gel-entrapment (Lenigk et al., 2000).

Alzheimer’s disease (AD) is a very serious pathological disease. Any insights that may be gained into the further progression of AD will lead to significant management, control of, and alleviation of this very serious debilitating disease. Different approaches may be used, and are being aggressively researched and applied to better understand this disease. The attempt made in this presentation to better understand the binding kinetics of AD biomarkers to different biosensor surfaces is a step in this direction.