(626u) Preperation of Steviol with Specific Enzymatic Hydrolysis of Stevioside Using b-Galactosidase From Sulfolobus Solfataricus

Abstract: Steviol is a
rare component in extract of Stevia rebaudiana Bertoni leaves, which
could be prepared by acidic hydrolysis or enzymatic hydrolysis of stevioside. b-Galactosidase
from sulfolobus solfataricus was found to hydrolyze all the glycosidic
bonds of St. The highest St conversion reached 99.0% in 6h with steviol as the
only product. By contrast, the mixture of steviol and isosteviol was obtained
by HCl hydrolysis. Therefore, enzymatic hydrolysis of stevioside by b-galactosidase
from sulfolobus solfataricus is a efficient process for selective
preparation of steviol.