(50g) Strategy Development for Cost Effective Lipid Production From Starchy Waste

Strategy
development for cost effective lipid production from starchy waste

Ramalingam Subramaniam, Stephen Dufreche, RakeshBajpai, and Mark E. Zappi

Bioprocessing Research
Laboratory, Department of Chemical Engineering

University of Louisiana at
Lafayette, Lafayette, LA

In this work, the
different strategies like batch, discrete feed, fed batch and continuous mode
of operation for the production of microbial lipids from sweet potato starch
were analyzed. The fermentation medium utilized in
this work has optimized concentrations of vitamins (Biotin, Calcium-Pantothenate and Inositol) in
place of yeast extract.  High lipid
yields were attained by providing an excess amount of carbon feedstock. Experiments
were conducted in a 5-L aerated, temperature, pH, and agitation
controlled fermentor. Batch and discrete batch experiments were conducted in
order to obtain the kinetic model. Fed batch and continuous processes were
explored for lipid production using the kinetic model. The results will be
presented at the meeting. In the production process, the oleaginous yeast Lipomyces starkeyi was utilized.