(594d) Semi-Industrial Production of Probiotic/Biotherapeutic Yeast Saccharomyces Boulardii in High Cell Density Culture Using Different Cultivation Strategies

Malek, R.A^1*, El Sayed, S², El Badry, I², Othman, N.Z¹, Sarmidi, M.R¹, Aziz, R.A¹,

El Enshasy, H.A^1,2

¹Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia

²Bioprocess Development Department, City for Scientific Research and Technology Applications (CSAT), New Burg Al Arab, Alexandria, Egypt

Corresponding author: roslinda@ibd.utm.my

Abstract

Saccharomyces boulardii is a yeast type probiotic strain and claimed to convey health benefits to hosts during consumption. This type of probiotic/biotherapeutic yeast is necessary to maintain human general health based on its immunological enhancing activities. This yeast is commercially available to consumers and already tested for its efficacy in preventing diarrhea and other Gastro-Intestinal (GI) disorders. Thus, this strain because one of the industrially important strain in probiotic and health industries. In the present work, cultivation of S. boulardii was carried out using different cultivation strategies to achieve high cell density cultivation with minimal alcohol production. Of different strategies applied, fed-batch cultivation of different modes were performed in 15-L stirred tank bioreactor. Of different feeding strategies applied, fed-batch cultivation using semi-defined medium as basal medium and fed with feeding solution composed of glucose, amino acids and vitamin exhibited maximal cell mass production reaching more than 80 g/L cell dry weight without significant alcohol produced in culture. In this work, this maximal cell mass produced was obtained using exponential fed-batch cultivation strategy, based on the constant values obtained from batch culture. The scalability of this cultivation strategy was also proved by further cultivation of this strain in 70-L stirred tank bioreactor using the same cultivation strategy.