(44g) Kinetic Analysis of Cytotoxicity With Chromonic Nanoparticles On Cancer Cell Lines: Comparative Analysis With Free Drug

Methotrexate (an anticancer drug) and folic acid (chromonic material) bears structural similarity, folic acid nanoparticles have been designed as mimicking drug molecules.The aim of this study is to compare the cytotoxic effect of nanoparticle-based anticancer drug with that in free drug form on cancer cell lines HepG2, Huh7 and HeLa. Folic acid. We investigated the effect of methotrexate and folic acid using different cancer cell lines. The proliferation of the cell line was found to be inhibited in a dose dependent manner which was detected using MTT assay. It was observed that the cytotoxic effect of the methotrexate and folic acid was higher and in controlled fashion in nanoparticle form than in the case of direct dosage. Kinetic studies revealed the sustained and controlled release patterns of the anticancer drug. Based on the results obtained, it can be envisaged that the in vivo use of nanoparticle based drug delivery and targeting is a promising approach in chemotherapy, overcoming the toxic effects of the known aqueous drugs.