(587o) Laccases Production By Pleurotus Ostreatus Grown On Grass Panicum Maximum

LACCASES PRODUCTION BY PLEUROTUS OSTREATUS GROWN ON GRASS PANICUM MAXIMUM

Morales Fonseca, Diana* ¹,Velásquez, Mario ¹

1.       Department of Chemical Engineering, School of Engineering, Universidad Nacional de Colombia, Bogota, Colombia.

* Corresponding author: dmmoralesf@unal.edu.co

Laccases are multi-copper enzymes with catalytic activity on oxidation of a broad range of phenolic and aromatic compounds, they have become a promising alternative for some industrial activities with potential environmental impact. Pleurotus ostreatus biomass used in enzymatic production was obtained during five days from wheat bran liquid culture medium. Laccases production was evaluated in submerged fermentation with the obtained biomass and Panicum maximum (5%w/v). Enzyme production was carried out with peptone, ammonium carbonate and ammonium sulphate at pH 4.5, 6.0 and 7.5, and three different carbon to nitrogen ratio (C/N) in the culture medium (10, 20 and 30). The highest activities (4111,82 U/L) were obtained with peptone at the following conditions: pH 6.0 and 10 C/N after six days incubation at 30°C and 150 rpm. This process could be proposed as a biological pretreatment to produce bioethanol from P. maximum and the use of laccases (in situ ) in detoxification of biomass hydrolysate.