(601c) Elucidation of Time and Bed Height Dependent Stationary Phase Intra-Particle Concentration Profiles in a Chromatographic Column

 Elucidation of Time and Bed Height Dependent Stationary Phase Intra-Particle Concentration Profiles in a Chromatographic Column

Ahmet R. Özdural

 Chemical Engineering Department, Hacettepe University, 06800 Ankara, Turkey

ABSTRACT

A simulation program has been developed for the prediction of intra-particle solute concentration profiles in chromatographic separations, where multicomponent analysis under nonlinear isotherm conditions is made. The validity of the assumption of local equilibrium (LE) conditions is uncertain in the dynamic state in a chromatographic column [1]. Therefore General Rate (GR) model is employed, where external mass transfer and intraparticle transport are included as separate identities. Thus the time taken for the solute to equilibrate between the stationary and mobile phases is taken into account for the prediction of the resulting band shape of a chromatographic peak.

The model presented in this work allows gaining an insight on the concentration gradient within a stationary phase particle.  Moreover the model illustrates how the intra-particle concentration gradient is affected with column height, time, mobile phase flow rate, mobile phase concentration, sample volume, sample concentration, particle diameter, as well as isotherm properties. It is shown that the methodology presented in this work allows quantitative investigations of solute adsorption dynamics within a single stationary phase particle of a column. This adds a new tool to the available methods for characterizing and optimizing chromatographic processes. 

 Reference:

[1]        Özdural A.R. (2011) Modeling Chromatographic Separation. In: Murray Moo-Young (ed.), Comprehensive Biotechnology, Second Edition, volume 2, pp. 681–695, Elsevier.