(792c) Post-Translational Modification of Artificial Cellulosomes for Efficient Cellulose Hydrolysis

Efficient hydrolysis of cellulose to glucose is gaining momentum because of its significant role in biofuel production. Cellulosome is a naturally occurring multi-enzyme system with a substantially enhanced ability for cellulose hydrolysis due to the effect of substrate targeting and enzyme clustering. The major component of cellulosome is a structural scaffoldin that consists of at least one cellulose binding module (CBM) and repeating cohesin domains that are docked individually with an enzyme tagged with a corresponding dockerin domain. Although artificial scaffoldins containing three or four orthogonal cohesins have been constructed, larger designer scaffoldins tend to aggregate and degrade. In this talk, we will highlight our recent strategies in engineering more complex artificial cellulosome structures through post-translational recombination for complex artificial cellulosome construction. The effect of enzyme loading on the overall enhancement in cellulose hydrolysis will be discussed.