(802g) Development of Aqueous Two-Phase Processes for the Recovery of High Value Biological Products

There is an increasing need to establish selective and scalable methods of product recovery and purification that integrate effectively with upstream operations. The established processes to purify biological products are characterized by a sequence of excessive membrane and chromatography steps. It is urgent to define bioengineering strategies to establish novel processes that can alleviate the existing process constrains for the recovery of biological products.

The development of novel purification processes based on two-phase extraction will be presented in this work. In particular, the fractionation of PEGylated proteins will be critically discussed. An attractive strategy for the recovery of recombinant proteins from plants, particularly rhG-CSF, from alfalfa will be examined. Finally, challenges for the establishment of bioprocesses for the purification of stem cells will be presented. Conclusions will be drawn concerning the strategies used to develop the downstream processes that greatly simplify the current way in which bioproducts can be recovered.