(331e) Extraction and Separation of Glucosinolates in Camelina sativa Seed Meal

Glucosinolates are anionic and hydrophilic secondary metabolites, which consist of several forms of sulfur-containing organic anions with β-D-thioglucose moieties, are present in the meal residue of Camelina sativa seed after oil extraction. In this study, anion exchange preparative chromatography (Q1 column and acidic aluminum oxide column) were used to separate the glucosinolates initially. Then the elution fractions were analyzed by reverse phase liquid chromatograph and mass spectrum. The results show that Q1 column and acidic aluminum oxide column can capture a group of glucosinolates from extracts. However, the Q1 column can achieve better selectivity of glucosinolates than aluminum oxide column. Moreover, KNO₃ solution is able to elute the absorbed glucosinolates from both columns. Mass spectrum was employed to analyze the elution from reverse phase Q1 column. The observed ions m/z 199, 241, and 284 are typical fragment of glucosinolate. Moreover, ion m/z 463 stands for 4-hidroxiglucobrassicin and ion m/z 409 stands for glucotropaeolin.