(256j) Enzyme-Polymer Conjugates Synthesized in Reverse Emulsions with Enhanced Activities

A simple approach for synthesizing enzyme-polymer conjugates is developed by using aldehyde-functionalized Pluronic F-127 as the reactive surfactant in the reverse emulsions. The applicability of this method was validated by using horseradish peroxidase (HRP), Candida rugosa lipase (CRL) and Candida antarctic lipase B (CALB) as model enzymes. Compared to native enzymes, the as-synthesized conjugate shows enhanced activity and stability in organic media and shows a great potential for applications in biocatalysis, biomedical and biosensing.