(280a) Quantitative Analysis of Viral Particles By Tunable Resistive Pulse Sensing Technology

Quantification of viral particles is a crucial issue in both virology research and biotechnology applications. However, characterization of viral particles remains challenging because of their nanoscale dimensions, as well as some limitations of conventional technologies. In recent years, tunable resistive pulse sensing technology has been developed rapidly as an emerging and useful tool for detection of individual nanoparticles. This study reports particle size distribution, concentration and zeta potential characterization of various viral particles, including tobacco mosaic virus, baculovirus, bacteriophage, and so forth. The results showed that the data of virusâ?? size was comparable to that from other measurement methods. The total viral particle concentrations were also obtained, which allowed comparison with previous infectious titer assay results. The zeta potential measurements provided additional surface charge information of viruses that has never been reported. This work enabled us to have an in-depth understanding of individual viral particles.