(280b) Multifunctional Fluorescent Optical Sensors for Bioanalysis
AIChE Annual Meeting
2016
2016 AIChE Annual Meeting
Sensors
Biosensor Devices: Applications
Tuesday, November 15, 2016 - 8:44am to 8:58am
Optical sensors are important tools for cellular imaging and analysis, for probing cellular metabolism, for understanding pathways and biological and physiological processes, and for diagnosing diseases and cancers. We have developed several series of fluorescent optical sensors (pH, oxygen (O2), potassium ion (K+), and glucose sensors) suitable for intracellular and/or extracellular imaging and analysis.
We integrated individual sensors as dual sensors using polymeric synthesis techniques for simultaneous multi-parameter measurements. Of particular value for application in the complex biological environment, some of the sensors emit two or even three colors in response to the same excitation wavelength, enabling ratiometric measurements and providing superior measurement accuracy. For example, we have prepared a dual pH/O2 sensor with three emission colors. The tri-color sensor composed of a blue emitter as an internal build-in reference probe, a green emitter as the pH probe, and a red emitter as the oxygen sensor. The build-in reference probe is unresponsive to pH or O2. It was demonstrated that this polymer-based tri-color sensor is capable for measuring the changes of pH and oxygen concentrations in photosynthesis cyanobacteria possessing high autofluorescence. We also integrated glucose and oxygen sensor as ratiometric dual glucose/oxygen sensors for analysis the glucose and oxygen consumption for bacterial cells and mammalian cells in real time. This dual glucose/oxygen sensor enables simultaneous measurement of glucose and oxygen, providing important information for continuous monitoring of glucose, diagnosing diabetes and hypoxia (low oxygen) related diseases and cancer, as well as the understating of biological processes of the metabolism.
We integrated individual sensors as dual sensors using polymeric synthesis techniques for simultaneous multi-parameter measurements. Of particular value for application in the complex biological environment, some of the sensors emit two or even three colors in response to the same excitation wavelength, enabling ratiometric measurements and providing superior measurement accuracy. For example, we have prepared a dual pH/O2 sensor with three emission colors. The tri-color sensor composed of a blue emitter as an internal build-in reference probe, a green emitter as the pH probe, and a red emitter as the oxygen sensor. The build-in reference probe is unresponsive to pH or O2. It was demonstrated that this polymer-based tri-color sensor is capable for measuring the changes of pH and oxygen concentrations in photosynthesis cyanobacteria possessing high autofluorescence. We also integrated glucose and oxygen sensor as ratiometric dual glucose/oxygen sensors for analysis the glucose and oxygen consumption for bacterial cells and mammalian cells in real time. This dual glucose/oxygen sensor enables simultaneous measurement of glucose and oxygen, providing important information for continuous monitoring of glucose, diagnosing diabetes and hypoxia (low oxygen) related diseases and cancer, as well as the understating of biological processes of the metabolism.