(518a) Proteomics Analysis of Antibody Producing CHO DG44 Cell Lines in Different Fed-Batch Processes

Fed-batch cell culture process is the most dominate operation mode for the production of recombinant therapeutic proteins. However, the limited systems biology understanding of the effect of different fed-batch processes on cellular mechanisms has hampered the effective process engineering for high protein productivity and quality. In this study, four basal media, four concentrated feeding supplements, and different feeding schedules were studied. The CHO DG44/IgG1 cell line produced high volumetric titer (1.3 g/L) and cell growth (19.6x10⁶ cells/mL). The comparative proteomics was performed using gel LC-MS/MS to analyze the host cell protein expression in different fed-batch processes. The CHO DG44 host cell and CHO DG44/IgG1 cultivated in both batch and simple fed-batch processes were used as controls. The expression levels of the proteins associated with transcription, translation, post-translational modification, carbon metabolism and cell growth were highlighted and investigated. In addition, the effect of glucose, glutamine, lactate, and amino acids were also evaluated. This proteomic study enabled the identification of the potential regulators of cell culture process engineering and cell engineering.