(584f) Optimization and Characterization of Zingiber Officinale Oil Encapsulated Nanostructured Lipid Carrier By D-Optimal Mixture Design

This study presents the application of the mixture design technique to develop an optimal nanostructured lipid carrier encapsulated Zingiber officinale oil (NLC-ZO). Eleven NLC mixture formulations were generated by D-optimal design technique and prepared using ultrasonication technique. The mixture formulations were generated by D-optimal design and independent factors were determined as percentage of Zingiber officinale oil (X₁), glyceryl monostearate as solid lipid (X₂), and virgin coconut oil as liquid lipid (X₃). The NLC-ZO formulation was characterized with respect to particle size, polydispersity index (PDI), zeta-potential and encapsulation efficiency as dependent variables. The optimum NLC-ZO has 0.36% Zingiber officinale oil, 0.14% solid lipid and 0.50% liquid lipid. Under the optimized condition, NLC-ZO formulation had an average diameter of 90.71 nm, PDI of 0.147, zeta-potential of -45.7 mV, and encapsulation efficiency of 88.80%. Encapsulation of Zingiber officinale oil was found enhancing penetration across Strat-M membrane due to its nano size and stability compared to non-encapsulated Zingiber officinale.