(683f) Catalase-Laden Microdevices for Cell-Mediated Enzyme Delivery

Catalase-Laden
Microdevices for Cell-Mediated Enzyme Delivery

Junfei Xia¹, Zhibin Wang¹, Yuanwei
Yan¹, Zhijian Cheng², Li Sun², Yan Li¹,
Yi Ren², Jingjiao Guan^1,*

¹ Department of Chemical and
Biomedical Engineering, FAMU-FSU College of Engineering, Florida State
University

² Department of Biomedical Sciences,
College of Medicine, Florida State University

Abstract

Enzymes have
been used as drugs to treat a wide variety of human diseases and traumas. However,
therapeutic utility of free enzymes is generally impeded by short circulation
time, lack of targeting ability, immunogenicity, and inability to cross certain
biological barriers. Various strategies have been developed to overcome these
limitations. Among them is cell-mediated drug delivery, which is featured by
integrating drugs with live cells and taking advantage of the unique
capabilities of the cells to achieve controlled drug delivery. We developed
simple and inexpensive methods based on soft lithography and layer-by-layer
(LbL) assembly for fabricating disk-shaped microparticles termed microdevices
and generating the cell-microdevice complexes. [1, 2] We herein applied these
methods to the fabrication of enzyme-laden microdevices and corresponding
cell-microdevices complexes for the first time. We used catalase as a model
enzyme in this study. It is a water soluble protein with an isoelectric point
of 5.8.  It is thus negatively charged at pH 7. Poly(diallyldimethyl
ammonium chloride) (PDAC) was chosen here as a polycation to form a bilayer
with catalase. Catalase in the microdevices was catalytically active and active
catalase was slowly released from the microdevices. The catalase-laden
microdevices were attached to the external surface of live suspension and
adhesion cells respectively to form cell-microdevice complexes. This technique
is applicable to other therapeutic enzymes and therapeutic cells, and thus
promises to find clinical applications for treating various human diseases and
traumas.

References

1.   
Junfei Xia,
Zhibin Wang, Danting Huang, Yuanwei Yan, Yan Li, Jingjiao Guan*,Asymmetric
Biodegradable Microdevices for Cell-Borne Drug Delivery, ACS Applied Materials &
Interfaces (2015) 7:
6293-6299.

2.    Zhibin
Wang, Junfei Xia, Yuanwei Yan, Ang-Chen Tsai, Yan Li, Teng Ma, Jingjiao
Guan*,Facile functionalization and assembly of live cells with
microcontact-printed polymeric biomaterials, Acta Biomaterialia (2015)
11: 80-87.