(103e) Characterization of  Dielectrophoretic Response of Candida Cells Using 3D Carbon-Electrode Dielectrophoresis

Here we present preliminary results
towards the characterization of the dielectrophoretic spectrum of different Candida cells. In the last two decades, Candida infection has emerged as the fourth
most common cause for morbidity and mortality in hospitalized patients in the
United States [1]. Five Candida species have been found to
be responsible for ninety percent of the all the cases of Candida infection: Candida albicans, Candida glabrata, Candida tropicalis, Candida krusei, and Candida parapsilosis [2]. The
current diagnosis of Candida infection begins with the culturing of tens of
milliliters of blood from the patient, which usually takes several days for
detection. During the time waiting for culture results, infection may become
progressively worse, leading to complications in treatment or death. These
complications can be exacerbated by beginning
treatment regimens that may not be effective for the particular Candida species causing the infection. Hence,
there is a need for rapid, species-specific detection of infection from Candida cells. Dielectrophoresis (DEP) is a
promising technique for the isolation and detection of the Candida cells within a few hours. Therefore,
it is important to characterize the DEP spectrum of these cells to understand how
DEP parameters (i.e. frequency, voltage, signal waveform) may contribute to the
degree to which cells can be trapped and separated for
detection. To date, we have characterized the frequency dependent
behavior of Candida albicans, Candida tropicalis, and Candida parapsilosis.

Strains of C. albicans, C. tropicalis, and C. parapsilosis were cultured in Yeast
Malt Broth (YMB) supplemented with a 40% glucose
solution at a 1:100 glucose to YMB ratio. Citric Acid was
added to the YMB media to adjust the pH to 3.5. Cells were
grown in suspension and dynamic incubation (shaking at 215 rpm) at 28°C for two days. For DEP
experiments, the experimental media was prepared for cell suspension and buffer
solution by dissolving 15% sucrose, 0.3%
dextrose, and 0.1% bovine serum albumin (BSA) in DI water. The media conductivity was 12.62 µS/cm. We prepared the
experimental cell suspension by suspending 300 µl of the cell culture in 5 ml of
the experimental media followed by washing and re-suspending in experimental media. The cell concentrations of the cell suspensions
were in the order of 10⁷ cells/ml. The fabrication of the carbon-electrode DEP device has
been detailed by our group in previous publications[3]. We flowed 58 µl of cell suspension
through the electric field of a sinusoidal signal of constant 20 Vpp magnitude and varying frequencies ranging from 10 kHz to 1 MHz. 100 µl of clean buffer was flowed through the DEP chip
at a constant flow rate of 10 µl/min to wash any trapped cells. We recorded the
release of the cells at the end of the electrode arrays upon turning off the
signal. Subsequent image analysis was completed with ImageJ, an image-processing software, to measure the
average intensity throughout the cell elution. The results from the image
analysis (Figure 1a) show the positive DEP response of cells with different
frequency. Figure 1b, 1c and 1d shows the C. albicans, C. tropicalis and C. parapsilosis cells trapped
on the carbon electrodes under positive DEP, respectively.

Ongoing work includes characterizing
the DEP spectrum of C. glabrata and C. krusei. We intend
to find their cell-wall capacitance from these DEP spectra so that they can be used in any numerical study involved the Candida cells. Future work includes
selectively isolating and concentrating any Candida species from other Candida species using DEP.

References:

ADDIN Mendeley Bibliography CSL_BIBLIOGRAPHY [1]      D.R. Andes, N. Safdar, J.W. Baddley, G.
Playford, A.C. Reboli, J.H. Rex, J.D. Sobel, P.G. Pappas, B.J. Kullberg, Impact
of treatment strategy on outcomes in patients with candidemia and other forms
of invasive candidiasis: A patient-level quantitative review of randomized
trials, Clin. Infect. Dis. 54 (2012) 1110–1122. doi:10.1093/cid/cis021.

[2]      S. Chakravarthi, N. Haleagrahara, A
comprehensive review of the occurance and management of systematic candidiasis
as an opportunistic infection, Microbiol. J. 1 (2011) 1–7.
doi:10.3923/mj.2011.1.7.

[3]      M. Islam, R. Natu, M.F. Larraga-Martinez,
R. Martinez-Duarte, Enrichment of diluted cell populations from large sample
volumes using 3D carbon-electrode dielectrophoresis, Biomicrofluidics. 10
(2016) 033107. doi:10.1063/1.4954310.