(191ar) Improved Production of Small Molecule Compound Though E. coli Metabolic System Optimization

Metabolic engineering provides an opportunity to access the small molecule compounds with superior benefit to disease therapy and environment protection but of low production and impurity in nature. Different from the normal biosynthesis pathway build-up in which multi plasmids bearing the specific genes are roughly introduced to a heterologous host as Escherichia coli, we enhanced and simplified biosynthesis pathway by integrating all these genes into only one plasmid and realizing the relative biosynthesis in the optimal host chosen from a number of candidates. The optimizaed synthesis system enables a consecutive gene translation, homogeneous production microenvironment and efficient material transportation which promise a highly-improved mechanism. Based on the study, we succeeded in boosting production of a plant-based anti-inflammatory agent salicylate 2-O-β-D-glucoside (SAG) and a nonribosomal peptide-polyketide siderophore Yersiniabactin (Ybt).