(194l) Optimization of Aqueous Extraction Conditions of Tannin from Quercus infectoria Galls

 Harisun,Y^1,2*., Iylia Arina,M. Z², Norazwani Z².,Siti Norazlina ,A.R².,Norfarahiyah A.N².,Norasiah S².

¹Department of Bioprocess Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor

²Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Skudai, Johor

*Corresponding author.

Email: harisun@ibd.utm.my

Abstract

Quercus infectoria is one of the greatest medicinal plants containing polyphenolic compound that has been reporting effective in possessing antibacterial, antifungal, antioxidant and many other biological functions. However, scientific literatures to support these biological actions are not well documented and to date there is no establish studies have been performed on the optimization of extraction conditions of Q. infectoria galls. The aim of this study is to optimize the extraction conditions of tannin to attain better yield of this marker compound from Q. infectoria galls. As such, Response Surface Methodology (RSM) was employed to optimize the extraction of Q. infectoria galls using aqueous decoction method based on Central Composite Design and was subjected to High Performance Liquid Chromatography (HPLC) for tannic acid quantification. The extract was also tested for its antioxidant activity using DPPH assay. From the HPLC analysis, tannic acid was indicated from 1149.11 mg/L to 3332.02 mg/L. Results also revealed that the optimum conditions for the extraction of Q. infectoria are at solvent to raw material ratio (23:1), extraction time (179.42 min) and extraction temperature (78.06⁰C) with yield of extract (82.03%), tannic acid content (3038.68 mg/L) and scavenging activity (90.76%). From this study, it was found that the Central Composite Design of experiment succeeded in the optimization of the extraction processing parameters of Quercus infectoria