(359d) The Mechanism of  Engineered Biofilms of Functional Bacteria on Root Surfaces for Organic Contamination Control and Soil Remediation

Organic chemical contamiantion has been a big threaten to soil. Current methods have some limitations. We developed a microbiological method to remove contaimation in soil, as well as to obtain the green cycle without further cotamination. Numerous studies revealed that decreased degradation of the second messenger cyclic di-GMP (c-diGMP) leads to the accumuation of biofilms. The goal of this study was to develop a new self-mediated method for biofilm to control their own formation on soil surface. First, the engineered plasmids and strains will be constructed with DNA recombination technique to modulate intracellular levels of c-di-GMP, which has been shown to regulate biofilm formation and dispersal in many bacterial strains. Further, EPS enhanced genetic traits from engineered strains will be spreads to other microbes in soil by plasmid transfer and adjusting expression level of c-di-GMP related proteins. A developed non-invasive quantification method based on imaging analysis from Confocal Laser Scanning Microscopy will be used to analysis biofilm formation on soil. As of our best knowledge, this is the first time that a biofilm self-mediated approach was used for bioremendaiton of orgainic contamination in soil. This system will become a flexible platform for various bioengineering purpose.