(760e) In vitro Study of a Drug Delivery Systems Constituted By Levan and 5-Fluorouracil with Different Cancer Cell Lines with an Overexpression in GLUT5

Levan is a fructose polymer that shows a great potential for biomedical applications due to its amphiphilic character and its affinity for a fructose transporter (GLUT5). This transporter is overexpressed in some cancer types, such as liver, colon or breast. However, drug delivery systems constituted by levan and a pharmaceutical have not been tested in vitro with the previous cancer cell lines. Based on the previous facts, we developed a drug delivery system levan and 5-fluorouracil and we tested in vitro its effect in different cancer cell lines with an overexpression in GLUT5 (liver, colon and breast cells). We obtained nanoparticles levan-5-fluorouracil of about 200-400 nm with a Z-potential value of -25 mV and in vitro results showed that levan can be used as a carrier for this purpose, decreasing the cell viability. Besides, levan without the drug also decreases the cell viability, showing a greater effect for colon cancer cell lines (cell line with the higher GLUT5 overexpression). According to the results, this biodegradable polymer can be used without a drug to treating cancer cell lines with overexpressed GLUT5.