(771f) Probing Phase Transitions in Dynamic Biopolymer Complexation
AIChE Annual Meeting
2017
2017 Annual Meeting
Materials Engineering and Sciences Division
Biomimetic Materials II: Applications
Friday, November 3, 2017 - 9:30am to 9:48am
Membraneless organelles formed in vivo and in vitro are strikingly similar to polyelectrolyte complexes formed using much simpler synthetic polyelectrolytes and charged homopolymer polypeptides (e.g., (poly)-lysine and (poly)-glutamic acid). In light of these observations, we designed a series of synthetic polypeptide sequences that mimic IDRs to elucidate the forces that govern membraneless organelle formation. Using solid-phase synthesis we are able to control the polypeptide sequence precisely to create alternating, block and gradient polypeptide copolymers to systematically investigate the influence of charged, polar and aromatic groups on phase separation using a variety of techniques. Our work holds the potential to develop a fundamental understanding of complex biopolymer complexation, impacting design of novel functional biomimetic materials for various applications.