(127e) A Synthetic Biology Platform for Flexible and on-Demand Drug Manufacturing

In this work, we developed a consolidated biomanufacturing platform to concurrently produce single or multiple biologics in single batches by the integration of inducible gene expression systems with upstream and downstream bioprocessing. First, we employed a recombinase-based gene integration system in Pichia pastoris for the rapid integration of large DNA payload into desired site on the genome. We then created three orthogonal small molecule-inducible systems for the inducible expression of single or multiple biologics in Pichia pastoris and showed the control over the ratio between co-produced biologic drugs. When combined with a portable microfluidic device, we were able to produce single dosages of protein drugs within 24 hours with limited infrastructure requirements. The synthetic biology platform offers a diverse array of options for on-demand, flexible, and decentralized biomanufacturing applications from personalized to industrial large-scale biologics production.