(188bn) Development of an Electrochemical Biosensor for Lactate Concentration Determination in Sweat

The purpose of this study is to find faster way to detect lactate concentration in human body instead of blood test. We develop a novel electrochemical biosensor to measure lactate concentration in human sweat. The lactate sensor has been successfully fabricated by using non-conductive nylon 6 membrane as the base of biosensor with adding functionalized multi-walled carbon nanotube for higher conductivity. Furthermore, eletrodeposition of lactate oxidase(LOx) enzyme along with pyrrole was carried out to detect lactate concentration. The aim of this work is to optimize the selectivity and storage stability for this sensor. The nanocomposite was characterized by scanning electron microscopy(SEM). To analyze sensor morphology, we use Fourier Transform Infrared Spectroscopy(FTIR) to verify the attachment of carboxyl group and LOx. Also will present the sensitivity of this sensor confirmed by cyclic voltammetry(CV), which shows oxidation peak compare to the control electrode.