(190s) Polydopamine Nanoparticles: A Possible Strategy to Fight Against Cancer
AIChE Annual Meeting
2018
2018 AIChE Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Poster Session: Engineering Fundamentals in Life Science
Monday, October 29, 2018 - 3:30pm to 5:00pm
Most in vitro biocompatibility studies are based on the accomplishment of a MTT assay, which relies on the absorbance measurement of the formed formazan salts in viable cellsâ mitochondria [3]. Nevertheless, polydopamine presents a great ability of adhesion to multiple materials [4], being the one that is used in culture plates found among them. As this polymer is able to absorb between 200 and 900 nm wavelengths, its absorbance overlaps with the formazan salts one and this fact could lead to a cell survival overestimation. So, what is the polydopamine real effect over cellular viability?
In order to answer such question, a new protocol for the performance of MTT assays with this melanin analogue has been stablished in the present work. Following this protocol, polydopamine cytotoxicity over two different tumor cell lines (BT474 and HTC116) has been studied and, as result, it has been shown that polydopamine could act as a possible antineoplasic agent.
References
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[2] Liu Y, Ai K, Liu J, Deng M, He Y, Lu L. Dopamine-melanin colloidal nanospheres: an efficient near-infrared photothermal therapeutic agent for in vivo cancer therapy. Advanced Materials. 2013;25:1353-9.
[3] Berridge MV, Herst PM,Tan AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Review: Elsevier; 2005. p 127-52.
[4] Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science. 2017;318:426-30.