(191an) Adapted Evolution and Biosensor-Based Screening for Robust Growth of Pseudomonas Putida on Corn Stover Hydrolysate and Cis,Cis-Muconic Acid Production

Despite the advancements in synthetic biology tools, our capacity to assemble DNA parts for engineering novel pathways cannot be matched to the complexities of biological systems. Adaptive laboratory evolution for superior genotypes and phenotype physiology coupled with screening for superior variants using target metabolite biosensor can be a powerful technique to eliminate the bottlenecks in the engineering of biosynthetic pathways. In the search for alternate sustainable technologies for making products originally sourced from petroleum, lignocellulosic biomass are an abundant renewable source of sugars for microbial production of bio-based commodities. Unlike from starch, they do not compete with food supply as well. In the current study, cis,cis-muconic acid (ccMA) producing strain of Pseudmonas putida was continuously grown on sugar hydrolysate of corn stover and the resulting diverse population was screened using ccMA sensor for variants with enhanced growth and product titers.