(198ar) Spinning-disc confocal microscopy in the second near-infrared window
AIChE Annual Meeting
2018
2018 AIChE Annual Meeting
Nanoscale Science and Engineering Forum
Poster Session: Nanoscale Science and Engineering
Monday, October 29, 2018 - 3:30pm to 5:00pm
Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000-1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as minimal or complete absence of tissue autofluorescence within this range, offers increased resolution and contrast with even greater penetration depths. Here, we present a custom-built spinning-disc confocal laser microscope (SDCLM) that is specific to imaging in the NIR-II. The SDCLM achieves a lateral resolution of 0.5±0.1 µm and an axial resolution of 0.6±0.1 µm, showing a ~17% and ~45% enhancement in lateral and axial resolutions, respectively, compared to the corresponding wide-field configuration. We furthermore showcase several applications that demonstrate the use of the SDCLM for in situ, spatiotemporal tracking of NIR particles and bioanalytes within both synthetic and biological systems.