(398a) Dynamic Modulation of Protein Functions by Strand Displacement

Inspired by the remarkable ability of natural protein switches to sense and respond to a wide range of environmental queues, here we report a strategy to engineer synthetic protein switches by using a generalizable strand displacement format to dynamically organize proteins with highly diverse and complex architectures. We showed that both DNA and coiled-coil peptide strand displacements can be used to dynamically control the spatial proximity and the corresponding functions of different proteins for both in vitro and in vivo applications. The general utility of the strategy was used to performing Boolean logic operations to provide explicit control of protein function using multi-input, reversible, and amplification architectures suitable for a wide range of applications.