(519a) Polymeric Microparticles as a New Platform for High Removal of Lipopolysaccharides
AIChE Annual Meeting
2018
2018 AIChE Annual Meeting
Separations Division
Membranes for Bioseparations
Wednesday, October 31, 2018 - 12:30pm to 12:50pm
Lipopolysaccharides (LPS) appear to be a major drawback for the production of biomolecules
that are essential for research, pharmaceuticals and industrial applications. Although there are a variety of methods for removing LPS, the question about how LPS removal can be carried out in an economical way is the most intriguing question without a satisfactory solution yet. High capacity per unit volume of adsorbents is a consideration for LPS adsorption. Here we demonstrate solid polymeric poly-e-caprolactone (PCL) microparticles (MPs) (ð‘‘" = 25.5 ± 10 ðœ‡ð‘š) as LPS binding sites by adsorbing these molecules on the surface of MPs. The PCL MPs removed ~82% LPS from water using only 1 milligram (mg) of MPs which is equivalent to ~2.22 × 10. endotoxin units (EU) per mg of particles. The LPS removal efficacy was relatively higher (~98%) in presence of phosphate buffered saline (PBS; 150 mM NaCl) than in water. An even more effective way was found to be the combination of MPs with a supporting layer such as a cellulose membrane that speeds up the LPS sorption process up to ~100% just by running the LPS containing water flow through it under gravity conditions. The water fluxes through the membrane were measured approximately 25 and 15 L/m2.h without and with MPs, respectively by gravity flow. A low ratio (~4.6) between the membrane thickness (~116 μm) and particle diameter (~25 μm) allowed convective solute transport that essentially enhances LPS adsorption within short residence times. The capacity per mg of MPs in the membrane was ~2.78 × 10. EU which was comparable to the adsorption capacity of MPs alone. The results indicate that PCL MPs have potential applications as LPS adsorbers in powder and membrane forms both in terms of high binding capacity as well as mass transport characteristics.
that are essential for research, pharmaceuticals and industrial applications. Although there are a variety of methods for removing LPS, the question about how LPS removal can be carried out in an economical way is the most intriguing question without a satisfactory solution yet. High capacity per unit volume of adsorbents is a consideration for LPS adsorption. Here we demonstrate solid polymeric poly-e-caprolactone (PCL) microparticles (MPs) (ð‘‘" = 25.5 ± 10 ðœ‡ð‘š) as LPS binding sites by adsorbing these molecules on the surface of MPs. The PCL MPs removed ~82% LPS from water using only 1 milligram (mg) of MPs which is equivalent to ~2.22 × 10. endotoxin units (EU) per mg of particles. The LPS removal efficacy was relatively higher (~98%) in presence of phosphate buffered saline (PBS; 150 mM NaCl) than in water. An even more effective way was found to be the combination of MPs with a supporting layer such as a cellulose membrane that speeds up the LPS sorption process up to ~100% just by running the LPS containing water flow through it under gravity conditions. The water fluxes through the membrane were measured approximately 25 and 15 L/m2.h without and with MPs, respectively by gravity flow. A low ratio (~4.6) between the membrane thickness (~116 μm) and particle diameter (~25 μm) allowed convective solute transport that essentially enhances LPS adsorption within short residence times. The capacity per mg of MPs in the membrane was ~2.78 × 10. EU which was comparable to the adsorption capacity of MPs alone. The results indicate that PCL MPs have potential applications as LPS adsorbers in powder and membrane forms both in terms of high binding capacity as well as mass transport characteristics.