(57g) (Keynote) Efficient Biosynthesis of Omega-3 PUFA: From Lab to Factory

As one of the three major nutrients, the consumption of lipid quality and quantity is closely related to the occurrence and development of lifestyle-related diseases chronic disease, such as cardiovascular and cerebrovascular diseases, obesity, and type 2 diabetes. WHO reported that dietary and nutritional factors are more effective approaches on health than medical factors. Omega-3 fatty acids, especiallay eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are critical for human health. It is estimated that human should have an omega-6:3 PUFA ratio of 1:1. However, current Chinese diet have an imbalance omega-6:3 ratios of 20 or even high. Marine fish are traditionally known as a natural source of EPA and DHA. However, the limitations of this source, such as seasonal availability, the ecological impact of fishing and the resulting environmental pollution, make it difficult to fulfill the nutritional requirements of human beings. In the past years, we have been conducting research work to engineer a sustainable land-based microalgae source of EPA and DHA to reduce pressure on fish stocks to meeting future demand. We isolated a genetic stability DHA producer industrial strain Schizochytrium sp. by high throughput screening method and the metabolic pathway analysis. Then, multi-index feedback cycle fermentation control technology and new bioreactors were developed to enhance lipid production. In parallel, a novel green lipid extraction technology has been developed that contains super-high levels of extraction, in the range of 90%-95%. These developments will provide theoretical basis and technological support for the industrial cultivation of oil-producing microorganism.