(175ad) N-Butanol Production from Lignocellulosic Biomass By Engineered Clostridium Cellulovorans

Butanol, as a promising biofuel, has excellent fuel properties and can be considered as a drop-in fuel without modifications of existing engines and fueling infrastructure.Lignocellulosic biomass is an abundant, cheap, and renewable source of carbon source, and thus is a desired feedstock for biofuels production. In our previous research, Clostridium cellulovorans, a natural cellulose/hemicelluloses utilizing,non-natural butanol producing bacterium, was metabolically engineered for n-butanol production directly from cellulose by overexpressing adhE2gene fromClostridiumacetobutylicum. In this study,low-cost lignocellulosic materialsincluding cotton stalk, sugarcane bagasse, soybean hull, and xylan were used for n-butanol production by engineered C. cellulovorans. In addition, we further studied the feasibility of C. cellulovoransto simultaneously use a mixture of glucose and xylose for n-butanol production. The results indicated that C. cellulovoransis a promising CBP platform host for bio-butanol production from cellulosic biomass.