(175b) Programming Animal Lipid Metabolism through Engineered Bacteria

Programming animal physiology hold great promise for agriculture, stock living, and human health applications. But animal physiology programming usually needs complicated, sometimes impossible, efforts. We present an engineered bacteria-based system to recognize external signals and respond by modulating animal fat metabolism. By putting genetic circuits in bacteria to control RNA and metabolites expression, we achieved programming model animal Caenorhabditis elegans physiology using “AND”, “OR” and “NOR” gates. They are achieved through orthogonal gene transfer or metabolites modulation. Using this platform we anticipated further interference of animal, including humans, metabolites and behavior correction for therapeutic, diagnostic and basic science applications.