(374a) Utilizing Gel Electrophoresis to Separate Aggreate after Photo-Crosslined Proteins
AIChE Annual Meeting
2019
2019 AIChE Annual Meeting
Separations Division
Poster Session: Bioseparations
Tuesday, November 12, 2019 - 3:30pm to 5:00pm
A key protein involved in Alzheimerâs disease is amyloid beta protein (Aβ). This disease effects more than 5 million US citizens to date. When amyloid beta starts to misfold and misbaheve, then the disease takes over. The misfolding of this protein leads to aggregation of proteins that cluster together to form different sized species. It has been researched that some of these have been shown to be neurotoxins. In order to properly treat the disease, understanding when these aggregations occur is crucial. By photo-crosslinking proteins, different aggregates can then be studied. Here we show that differences in size aggregates can be separated by gel electrophoresis after being photo-crosslinked to stabilize their forms.