(374c) Optimization of Hydrodistillation Extraction Process of Tea Tree Oil Using Response Surface Methodology
AIChE Annual Meeting
2019
2019 AIChE Annual Meeting
Separations Division
Poster Session: Bioseparations
Tuesday, November 12, 2019 - 3:30pm to 5:00pm
The
optimization of hydrodistillation extraction of tea tree oil (TTO), i.e. the
essential oil of Melaleuca alternifolia, was carried out with the
response surface methodology. Nonionic surfactants, Tween 20 and Tween 80, were
added in the still to help the solubilization of TTO to the liquor and,
consequently, to increase the extraction yield of TTO by the hydrodistillation
process. In principle, this study evaluated the relevance of several
independent parameters, including the surfactant concentration, the extraction
time, and liquid/solid ratio, with respect to TTO yield with a design of
experiment (DOE) based on response surface methodology. Central composite
design (CCD) was used to optimize the conditions of TTO extraction process. The chemical compositions
of tea tree oil were analyzed and quantized by GC-FID and were refereed with
the international standard ¡§ISO 4730¡¨. Additionally, TTO obtained from optimal
condition was examined on the stability of its microemulsion formulations as well
as the antibacterial property, compared with the commercial TTO.
optimization of hydrodistillation extraction of tea tree oil (TTO), i.e. the
essential oil of Melaleuca alternifolia, was carried out with the
response surface methodology. Nonionic surfactants, Tween 20 and Tween 80, were
added in the still to help the solubilization of TTO to the liquor and,
consequently, to increase the extraction yield of TTO by the hydrodistillation
process. In principle, this study evaluated the relevance of several
independent parameters, including the surfactant concentration, the extraction
time, and liquid/solid ratio, with respect to TTO yield with a design of
experiment (DOE) based on response surface methodology. Central composite
design (CCD) was used to optimize the conditions of TTO extraction process. The chemical compositions
of tea tree oil were analyzed and quantized by GC-FID and were refereed with
the international standard ¡§ISO 4730¡¨. Additionally, TTO obtained from optimal
condition was examined on the stability of its microemulsion formulations as well
as the antibacterial property, compared with the commercial TTO.
Keywords: Melaleuca alternifolia; Tea Tree Oil; Extraction;
Design of experiment (DoE); Response Surface Methodology (RSM)
Figure
1:
Effect of surfactant (Tween 20) concentration on the hydrodistillation
extraction kinetics of tee tree oil.
