(381an) Study on Recrystallization of Cephalosporanic Acid G

Abstract: The recrystallization of Cephalosporanic acid G in different solvents, such as methanol-water, ethanol-water, sodium bicarbonate-sulfuric acid, sodium bicarbonate-hydrochloric acid, ammonium bicarbonate-sulfuric acid, ammonium bicarbonate-hydrochloric acid, ammonia water-sulfuric acid, ammonia water-hydrochloric acid, was studied. The dynamic laser method was used to determined the solubility of Cephalosporanic acid G in ethanol, pure water and ethanol-water. In the mixed solvent of ethanol-water, the solubility of Cephalosporanic acid G was found to increase first and then decreased with the increase of the molar fraction of water, and reached the maximum when the molar fraction was 0.3.The mesostable region of Cephalosporanic acid G in ethanol-water system was determined. The thermodynamic model Apelblat equation, Van’t Hoff equation, lh equation, Wilson equation and NRTL equation were used to fit data. The interaction energy between Cephalosporanic acid G and different solvents was simulated with Materials Studio software to discuss the difficulty of forming hydrogen bonds. Based on the thermodynamic properties of Cephalosporanic acid G, the crystallization process of ethanol-water was designed. The effects of temperature range, crystallization time, flux acceleration rate and agitation rate on product purity and yield were studied by univariate method. The optimum technological conditions for solvent crystallization of Cephalosporanic acid G were determined.

Abstract: The recrystallization of Cephalosporanic acid G in different solvents, such as methanol-water, ethanol-water, sodium bicarbonate-sulfuric acid, sodium bicarbonate-hydrochloric acid, ammonium bicarbonate-sulfuric acid, ammonium bicarbonate-hydrochloric acid, ammonia water-sulfuric acid, ammonia water-hydrochloric acid, was studied. The dynamic laser method was used to determined the solubility of Cephalosporanic acid G in ethanol, pure water and ethanol-water. In the mixed solvent of ethanol-water, the solubility of Cephalosporanic acid G was found to increase first and then decreased with the increase of the molar fraction of water, and reached the maximum when the molar fraction was 0.3.The mesostable region of Cephalosporanic acid G in ethanol-water system was determined. The thermodynamic model Apelblat equation, Van’t Hoff equation, lh equation, Wilson equation and NRTL equation were used to fit data. The interaction energy between Cephalosporanic acid G and different solvents was simulated with Materials Studio software to discuss the difficulty of forming hydrogen bonds. Based on the thermodynamic properties of Cephalosporanic acid G, the crystallization process of ethanol-water was designed. The effects of temperature range, crystallization time, flux acceleration rate and agitation rate on product purity and yield were studied by univariate method. The optimum technological conditions for solvent crystallization of Cephalosporanic acid G were determined.