(655e) Synthesis of Oligosaccharides Directly from Mono- and Di-Saccharides As Potential Prebiotics in Inorganic Ionic Liquid

This study demonstrated a process for high-yield and high-selectivity synthesis of high-value oligosaccharides (such as glactooligosaccharides, glucooligosaccharides, fructooligosaccharides, and xylooligosaccharides) from low-cost mono- and di-saccharides from biomass and food industry (such as glucose, fructose, galactose, xylose, lactose, and sucrose) via glycosylation reaction in an inorganic ionic liquid, acidic lithium bromide trihydrate (ALBTH) solution. The synthesized oligosaccharides were characterized using high performance liquid chromatography, matrix-assisted laser desorption ionization-time of flight mass spectrometry, gel permeation chromatography, and nuclear magnetic resonance analysis for sugar profile, degree of polymerization (DP), and glycosidic bonds linked the sugar units. Preliminaryin-vitrofermentation with select probiotic strains verified the prebioticpotential of the synthesized oligosaccharides.