(669b) Cell Wall Disruption in Low Temperature By KOH/Urea and Potential Application of Its Liquid Waste in Crop Production

Cell wall disruption in
low temperature by KOH/urea and potential application of its liquid waste in
crop production

Xinshu Zhuang, Zahoor, Wen Wang, Xuesong
Tan, Qiang Yu, Zhenhong Yuan

Guangzhou Institute of Energy
Conversion, Chinese Academy of Sciences; CAS Key Laboratory of Renewable
Energy; Guangdong Provincial Key Laboratory of New and Renewable Energy
Research and Development, Guangzhou 510640, P. R. China

Abstract

 Pretreatment of corn straw by KOH/Urea
(KU) aqueous solution at low temperature was investigated. To study the cell
wall distraction during this low temperature process, and its impacts on enzymatic
hydrolysis, cellulose
crystallinity, and chemical properties were
investigated by High performance liquid
chromatography, Scanning electron
microscopy, Fourier transform infrared spectra, and X-ray diffraction. The
results showed that the degradation of plant cell wall at low temperature was
attributed to distraction of the hydrogen bonds in cellulose and solubilization
of hemicellulose and lignin. Meanwhile, the pretreatment approach resulted in
increasing cellulose recovery up to 29%, approximately 33% of lignin and 15% of
hemicellulose removal, respectively. Under KU pretreatments, biomass samples
exhibited much enhanced hexoses yields from enzymatic hydrolysis, a higher
saccharification (80%) of cellulose in pretreated materials was achieved with
domestic cellulase loading of 20 FPU/g substrates within 2h at 30 ⁰C.
Further co-supplied with 1% Tween-80 exhibited an almost complete enzymatic
saccharification of the
residues. It is interesting to note that the
pretreatment liquid waste (PLW) were applied as phytogenic molecular activator,
leading to increase dry biomass yields by 3¨C24 times compare to control in
different crops. Hence, this study has not only demonstrated a new pretreatment
technology for a complete saccharification, but it has also produce phytogenic
molecular activator for the crop growth through PLW without any negative impact
on environment, which laying the foundation for the cellulosic ethanol
bio-refinery.

Keywords:
Pretreatment, Lignocellulose, KOH/Urea, Cell wall disruption, liquid waste, phytogenic
molecular activator, crop production.

This
work was financially supported by the National Key Research and Development
Program of China (2018YFB1501402), the National Natural Science Foundation of
China (51876206,51606203,51476179).