(676d) Serum Effects on Nanoparticle-Mediated Photoporation for Enhanced Intracellular Drug Delivery
AIChE Annual Meeting
2019
2019 AIChE Annual Meeting
Nanoscale Science and Engineering Forum
Bionanotechnology for Gene and Drug Delivery II
Thursday, November 14, 2019 - 1:24pm to 1:42pm
This process has been optimized for >90% of cells exhibiting intracellular uptake of low-molecular weight (less than 1 kDa) marker compounds without significant cell viability loss. Understanding how changes in cellular micro-environment affect delivery efficiency is important for practical translation of this platform technology. Therefore, this study is focused on understanding the role of serum during photoporation, and how that understanding can be used to increase intracellular delivery of macromolecules.
Experimental results reveal 75% less loss of cell viability during laser irradiation at high fluence when cells are suspended in media containing 10 (v/v)% serum. Similar effects are observed in media containing albumin (major protein component present in serum) as well as denatured serum proteins. Further experiments show that a few polymer additives also help preserve cell viability during photoporation and thus viability protection by serum appears to be attributed to physical interactions between serum components and cell membrane/suspension media and not to biological activity, for example, associated with serum proteins. The ability of cells to stay alive at high fluence may enable macromolecular delivery (greater than 10 kDa) which have applications in protein and gene therapy. Thus, future experiments will explore these avenues in addition to investigating the mechanism behind cell viability protection in presence of serum and polymer additives.
Reference:
1. Sengupta et al. Efficient Intracellular Delivery of Molecules with High Cell Viability Using Nanosecond-Pulsed Laser- Activated Carbon Nanoparticles. ACS Nano 2889â2899 (2014)