(6g) Fabrication of Highly Sensitive LSPR Biosensor for the C-Reactive Protein (CRP) Detection Based on the Immunocolloidal Gold Nanoparticles

Biosensor technology for the rapid and sensitive detection of disease and pathogenic bacteria generally requires the sample preparation steps such as pre-concentration and desalting, and the process of labeling with fluorescence dyes and chemical reporters. Recently, there has been a growing interest in non-labeled biological detection techniques that can be detected without additional sample preparation steps. Here, we demonstrate a simple method for the fabrication of plasmonic-active chip which are uniformly deposited in a single Au layer. The glass based biosensor chip that can be effectively SPR driven was prepared via the control of the chemical ligand concentration, temperature change, and surface modification. As such, we examined the correlation between the enhanced plasmonic effect and surface structure of the resulting substrate to determine the optimum conditions for highly sensitive LSPR sensor chip. To confirm the possibility of the non-labeled LSPR biosensor, we apply it to the quantitative detection of C-reactive protein associated with cardiovascular and renal risk factors. We report a limit of detection for CRP of 1 ng/mL. Therefore, our LSPR biosensor chip could provide a significant contribution to the ongoing efforts to fabricate non-labeled biosensor chips for fields such as diagnostic, medical, and environmental and food.

Teaching Interests: Biosensors / Devices