The Study of Natural Wood Vinegar's Active Time for Antimicrobial Testing by Time-Kill Methods to Human Harmful Microorganism

Natural wood vinegar or natural pyroligneous acid is a by-product from charcoal making process. This thick dark brown natural liquid consists of more than 200 water soluble compounds such as phenolic, alkane, alcohol, organic compound, acetic acid and ester. In this study, natural wood vinegar application become a targeted natural active ingredient to contribute in biocosmetics, bioprocess and biopharmaceutical industry. In this study, five human harmful microorganism which related urinary tract infection caused: Candida albicans 10231, Escherichia coli 8739, Enterococcus faecalis 19433, Proteus vulgaris 33420 and Klebsiella pneumoniae 13883 were studied. Time-kill kinetics assay method was selected to study natural pyroligneous acid as antimicrobial agent to determine bacteriostatic and bactericidal activity. Variety of target active time (0- 240 minutes) of natural pyroligneous acid was tested. Each strain of antibacterial was quantified incubation at 37oC. The result showed that positively anti microorganism which related urinary tract infection caused during the targeted active time. The results of this research provide convincing evidence of the potential active ingredient in bio-wellness industry.