(102e) Advances in Dynamic and Responsive Materials for Biological Applications

Methods for culturing mammalian cells in a biologically relevant context are increasingly needed to study cell and tissue physiology, expand and differentiate progenitor cells, and to grow replacement tissues for regenerative medicine. Two-dimensional culture has been the paradigm for in vitro cell culture; however, evidence and intuition suggest that cells behave differently when they are isolated from the complex architecture of their native tissues and constrained to petri dishes or material surfaces with unnaturally high stiffness, polarity, and surface to volume ratio. As a result, biologists are often faced with the need for a more physiologically relevant 3D culture environment, and many researchers are realizing the advantages of hydrogels as a means of creating custom 3D microenvironments with highly controlled chemical, biological and physical cues. Further, the native extracellular matrix (ECM) is far from static, so ECM mimics must also be dynamic to direct complex cellular behavior. In general, there is an un-met need for materials that allow bio-responsive or user-defined control over the spatio-temporal presentation of biological signals, such as integrin-binding ligands, growth factor release, and biomechanical signals. Developing such biomaterial mimics of the ECM for 3D cell culture is an archetypal engineering problem, requiring control of numerous properties on multiple time and length scales important for cellular functions. New materials systems have the potential to significantly improve our understanding of how cells receive information from their microenvironment and the role that these dynamic processes may play in applications from controlling the stem cell niche and organoid growth to in vitro models of diseased tissues for drug screening. This talk will illustrate several examples of recent advances in biomaterial chemistries and processing for regenerative medicine applications, and how one can dynamically control biochemical and biophysical properties through orthogonal, photochemical click reaction mechanisms.