(123g) Antipathogenic Activities of Nitrogen, Sulfur, Boron Doped Carbon Dots

Nitrogen, sulfur, and boron-doped carbon dots (CDs) were prepared via the hydrothermal treatment of maleic acid (MA) as a carbon source with polyvinylamine (PVAm) as a nitrogen (N) source with cysteine (Cys) as a sulfur (S) source, and with boric acid as a boron (B) source. Different combinations such as N-, S-, B-, N/S-, N/B, N/S/B-doped CDs were synthesized and their antipathogenic capabilities on various microorganisms were examined. The blood compatibility by means of the hemolysis and blood clotting tests and the cytotoxicity on L929 fibroblast cells were determined to assess the biocompatibility of the prepared CDs. Finally, the antibacterial activities of each composition e.g., N-, S-, B-, N/S-, N/B, N/S/B-doped CDs were investigated against B. subtilis ATCC 6633 (Gram +), Staphylococcus aureus ATCC 6538 (Gram +), Escherichia coli ATCC 8739 (Gram -), P. aeruginosa ATCC 10145 (Gram -) and Klebsiella pneumoniae ATCC 700603 (Gram -) bacteria species via disc diffusion and microtiter dilution assays. Furthermore, biofilm inhibition and disruption ability of the most effective CDs were determined against Candida albicans ATCC 10231.

Acknowledgments: This work is supported by Scientific Research Commission of Canakkale Onsekiz Mart University FIA-2022-3667.