(188g) Removal of Allergens from Pistachio Shell By Soxhlet Method; An Experimental Study

Agricultural wastes are accounted as cheap and available enriched materials, which have been widely used in different applications such as fertilizers, composite structures. One of the most available agricultural wastes are pistachio shells, which are being disposed more than 500000 tons per year, in the United States. These wastes would be proper sources of animal feed. Allergens are found in different shell powder containing nuts, and could make some problems for animal and people dealing with the shells powder, if not extracted from the shell powder. By adhering to the surface of an immune cell with allergens, the secretion of Immunoglobulin E (IgE) occurs. This event triggers a cell signaling cascade, leading to an allergic response. Based on the literature, the allergens include the 2S albumin, 7S vicilin, and 11S legumin proteins, conglutin, and glycinin. In our experimental study, we identified the allergens of pistachio shell. Different methods of allergen removal, including rinsing with solvent, acid treatment, lights radiation processing, and Soxhlet extraction were investigated. The Soxhlet method was chosen (because it showed a selective removal), and pistachio proteins were extracted and separated by SDS-PAGE. The 7S vicilin-like protein was identified and extracted, as the equal code of pistachio shell Pis v 3.