(213h) High Sensitivity Saliva-Based Biosensor in Detection of Breast Cancer Biomarkers: HER2 and CA15-3

Breast cancer is the most commonly diagnosed cancer among women. Salivary biomarkers offer a non-invasive approach to detecting this cancer. In this study, a virus detection device based on metal-oxide-semiconductor field-effect transistors (MOSFETs) and functionalized, disposable test strips were utilized. The results demonstrated impressive limits of detection (LOD) and sensitivity when analyzing common breast cancer biomarkers, including HER2 and CA15-3. The LOD for these two biomarkers reached as low as 10^-15 g/mL, and the detection of HER2 exhibited a high sensitivity of approximately 70/dec, enabling differentiation between HER2-negative cases. The sensitivity of CA15-3 was 30/dec, which was also able to distinguish between healthy individuals and breast cancer patients. Furthermore, the test takes less than a minute to determine the actual concentration of biomarkers in saliva. The technique is easy to operate and has the potential for widespread public use in the future.