(337f) Bioprocess Development for Antimicrobial Production in Pseudomonas Protegens Emm-1

Upstream bioprocess development, fermentation and cell culture. Transcriptomics. Identificaction of metabolites by LC-MS.

Abstract.

Pseudomonas protegens EMM-1 is a highly competitive bacterium capable of producing one or several inhibitory substances. P protegens EMM-1 inhibits the growth of several multi-drug resistant pathogenic microorganisms like Klebsiella, Burkholderia, and Streptococcus. The identification of the antimicrobial compounds produced by P. protegens EMM-1 is of great importance because of the growing threat of pathogenic bacteria. In this work, the metabolic pathways and culture conditions that maximize the production of antimicrobial compounds produced by P. protegens EMM-1 will be presented. 2,4-Diacetylphloroglucinol and orfamide A were found in the culture broth. Culture extracts were successfully assayed against Streptococcus sp. SP6 and Klebsiella sp. isolated from clinical samples. The approach used in this work can be applied to other bacteria with the potential to produce antimicrobial compounds.