(560h) Isolation of Peptidoglycan from Filamentous Microorganisms for New Bioinspired Water-Responsive Materials
AIChE Annual Meeting
2023
2023 AIChE Annual Meeting
Materials Engineering and Sciences Division
Biomimetic Materials II
Thursday, November 9, 2023 - 5:36pm to 5:54pm
In this research we have cultivated the Oscillatoria tenuis cyanobacteria and isolated the biomolecular material peptidoglycan from this organism. We studied its water-responsive strain, response speed, and energy densities by using the atomic force microscope (AFM) that we previously customized for this purpose. We constructed peptidoglycan bioconjugates to aid controlling and positioning the biomaterial. This data was compared the extracted material with those from commercially available sources and also with our previous work with B. subtilus. In further studies we have used the biosynthetic uptake of fluorescent D-amino acids to label and characterize the 3D structure of fluorescent peptidoglycan using AIRYSCAN superresolution microscopy. We conclude that we can scale up the production of this novel, labeled biomaterial to appropriate levels for further use in water-reponsive devices and materials. We hypothesize that cell wall adaptations in the peptidoglycan due to the characteristics of species in the genra could result in biomolecular materials with more advantageous water-responsive actuation properties.