Experimental Design for Biomaterials Education: 3D Bioprinting
AIChE Annual Meeting
2023
2023 AIChE Annual Meeting
Annual Student Conference: Competitions & Events
Undergraduate Student Poster Session: Education & General Papers
Monday, November 6, 2023 - 10:00am to 12:30pm
In the IBL experiment, the main variable explored by students was altering the viscosity of the support bath, which impacts print fidelity of a bioink. Two types of commonly used support bath polymers were utilized in this module: gelatin hydrogels and Carbopol® 691 polymer jammed microparticles. Both polymers are safe and accessible. The module was designed to highlight the difference in the type and degree of secondary entanglements gelatin and Carbopol® 691 polymer have regarding their effect on support bath viscosity and printing. Moreover, a secondary variable students explored was altering the concentration of the bioink (gelatin containing Coomassie blue for visualization). In doing so, students can learn how bioink composition can affect printing fidelity.
Learning gains for the module will be assessed using a pre-/post-test with questions pertaining to the factors affecting viscosity in 3DBP printing fidelity. Additionally, a validated pre-/post-survey based on those from the Scientific Literacy and Student Value in Inquiry-guided Lab Survey (SLIGS) was given3. The 3DBP IBL educational module was designed for undergraduate students to have a hands-on way to explore how factors affect print fidelity.
References
[1] Huebsch, N.; Mooney, D. J. Inspiration and application in the evolution of biomaterials. Nature 2009, 462, 426â432 DOI: 10.1038/nature08601.
[2] Vanaei, S.; Parizi, M. S.; Vanaei, S.; Salemizadehparizi, F.; Vanaei, H. R. An overview on materials and techniques in 3D bioprinting toward biomedical application. Engineered Regeneration 2021, 2, 1â18 DOI: 10.1016/j.engreg.2020.12.001.
[3] Ankeny C, Stabenfeldt S. Cost-effective, Inquiry-guided Introductory Biomaterials Laboratory for Undergraduates. 2015 ASEE Annual Conference and Exposition Proceedings, ASEE Conferences; 2015, p. 26.412.1-26.412.16. doi:10.18260/p.23751.