Programmable Dynamic Self-Assembled DNA Nanostructures

In addition to storing genetic information, DNA serves as a distinct and predictable nanomaterial capable of self-assembling into precisely programmed structures. Among various DNA technologies, DNA nanostructures can be employed to create DNA nanorobots with the ability to perform various tasks, including molecular transport and release. Self-assembled DNA nanostructures emerge as excellent candidates to overcome the limitations of conventional drug delivery methods, thanks to their biocompatibility and programmability. Efforts have been focused on manipulating these structures to enhance their reconfigurability, leading to groundbreaking developments of structures that facilitate and cease nanotube growth.

In this project, we introduce a novel structure that combines both functions: nucleation and termination. By adjusting the structures dedicated to two individualized tasks, we achieve an integration of two functionalities into one structure. One end of the structure facilitates nanotube growth and the other end terminates growth. Consequently, segments of DNA nanotubes can connect, forming more sophisticated structures. Additionally, we investigated the effects of free monomer concentrations on nanotube growth. Concentrations play a pivotal role in nanotube growth dynamics, and an ideal growth curve encompasses successful nucleation of nanotubes followed by consistent, sustained growth. By observing nanotube growth under varying concentrations, we gain important insights that further optimize our nanotube system. This project significantly improves the programmability of our existing DNA nanotube systems and enhances our understanding of the optimal conditions for nanotube growth. The advancements are promising to contribute more to the DNA nanotechnology toolkit.