(83ae) A Thick Film Printed Electrochemical Biosensor and Its Application for Testing the Ketone 3-β-Hydroxybutyrate for Potential Diabetic Patient Management

The American Diabetes Association advises that blood ketone testing methods that quantify 3-β-hydroxybutyrate (3HB) will be desirable for the diagnosing and monitoring ketoacidosis for diabetic patient management. Blood ketone refers to 3-β-hydroxybutyrate (3HB), acetoacetate (AcAc) and acetone. The detection of 3HB is suggested for the management of DKA. Amperometric biosensors based on enzymes have raised increasing attention recently due to the high selectivity, sensitivity and convenience of these biosensors. Thick film printing technique has been applied to the fabrication of amperometric biosensors, improving the quality and reproducibility of the biosensors, and reducing the cost. In this study we can show that the detection method using this biosensor involved only a single reaction step without any mediator. The concentration of 3HB could be quantified with enzyme 3HBDH and NAD+ immobilized on the working electrode of this biosensor prototype (Figure 1). This electrochemical biosensor could be used effectively for 3HB detection for potential diabetic patient management. Fig.1. Amperometry of 3HB tested by biosensor under 37.5 ºC in the presence and absence of enzyme 3HBDH