(106f) CLEAN -up of OIL Spill by Oilzapper: Consortia of NOVEL BACTERIAL Strains A FIELD STUDY- Visakhapatnam
AIChE Spring Meeting and Global Congress on Process Safety
2012
2012 Spring Meeting & 8th Global Congress on Process Safety
Liaison Functions
Spring Meeting Poster Session and Networking Reception
Tuesday, April 3, 2012 - 5:00pm to 6:30pm
Clean -up of oil spill by oilzapper: consortia of novel bacterial strains a field study- visakhapatnam
Ankit lal, Bina Singh, Priyangshu. M. Sarma, Ajoy. K. Mandal, Banwari lal
Environmental and Industrial biotechnology
The energy research institute
Darbari Seth Block, Habitat Place
Lodhi Road-New Delhi-11003
ABSTRACT
Bioremediation has become an important method for the restoration of oil-polluted environments by use of indigenous or selected microbial flora. We have developed a consortium of five novel strains (Oilzapper); a field scale study was conducted in a land contaminated with an oily sludge by use of a carrier based hydrocarbon- degrading consortium for bioremediation. The land belonged to oil refinery; feasibility study was conducted prior to field study to access the potential of bacterial consortium to degrade oily sludge. At Visakhapatnum (India) pits were undertaken for bioremediation. In this study consortium was prepared in 1500L bioreactor (Bio-engineering) and along with nutrients mixture was used in field area. Samples of the soil at zero day (without application of microbial consortia) and after application of microbial consortia at different time interval period i.e. one month two and three months till complete degradation of oily sludge/ soil were collected. Soils collected from the sites were subjected for oil extraction using soxhlet method. Weight of the oil was calculated by simple gravimetric method. Column fractionation was done for the extracted oil to separate alkane and aromatic by hexane and toluene. Characterization of oil was done by (Gas Chromatograph (Hewlett Packard 5890 Series II using, GC Column (DB2887) for Alkane and Column (DB23) for Aromatic samples.
*Presenting author: Ankit lal
Research associate trainee
Environmental and Industrial biotechnology
The energy research institute
Darbari Seth Block, Habitat Place
Lodhi Road-New Delhi-11003
Email: ankitlal123@gmail.com
Ph. +91-11-24682100 (m) 09560156445 Fax 24682100